Steppe Ancestry definitely arrived in India post 1000 BCE - The Final Blow

कर्ण (karNa), by @zdrava

In my first post on this topic - 'The True source of steppe ancestry in modern Indians' - I laid out the genetic claim that the best source for steppe in Indians seems to be a sample from the Yaz II Iron Age culture site at Takhirbai 3, Turkmenistan (short name of the sample - TKM_IA), dated to approximately 850 BCE. Based on other archaeological, anthropological, epigraphic and literary evidence which also supported this claim, I proposed that only a post-1000 BCE movement of these 'proto-Śāka' people could explain the steppe ancestry present in modern Indians. The main reason to reject the view that steppe folks came to the core Vedic region (of Punjab, Haryana and East UP) around ~1500 BCE, and gave Indians the Vedic culture and Indo-European languages is the fact that absolutely no archaeological evidence exists to support such a big claim. This difference is important - a post-1000 BCE movement of Iranian-speaking proto-Śāka into India cannot bring the Vedic culture and language. Neither does it match the evidence from Rig Veda, which describes a pre-Iron Age life. Do read that article to understand all the evidence in support of my claim. 

The Lady from Central Asia who was found dead in a Bronze Age Turkish well

 

Site of the Alalakh 'Well Lady'. Courtesy: Skourtanioti et al 2020

The remains of the Alalakh 'Well Lady' were discovered in the archaeological site of Tell Atchana / Alalakh / Alalah, Turkey dating to ~1550BCE. Her aDna was published in 2020 (1) and she was found to be of central Asian origin, far from where she was found. She was found at the bottom of a deep well that was still in use at the time. The individual showed evidence of healed trauma on the skull's frontal bone and two healed fractured ribs, and her manner of death has been suggested as a homicide due to or before being thrown into the well (2). Her teeth also showed clear signs of enamel defects (2), a possible sign of malnutrition during childhood. Her age at death was 40-45 years.

The Southern Arc paper and it's data upends the Steppe Theory in multiple ways

Lazaridis, Iosif, et al. “The Genetic History of the Southern Arc: A Bridge between West Asia and Europe.” Science, vol. 377, no. 6609, 2022, https://doi.org/10.1126/science.abm4247.

This is a recently published paper ('the paper', 'this paper') with many new samples from what the authors call 'The Southern Arc'. The authors define it as 'a region centred on the large Anatolian peninsula (Turkey), including in the west (in Europe) the Balkans and the Aegean, and in the south and east, Cyprus, Mesopotamia, the Levant, Armenia, Azerbaijan, and Iran.'

The main conclusion of this paper which upends one part of the Steppe theory is that West Asia is now considered to be the homeland of the first Indo-European speakers, and the steppe to be only a secondary homeland. From the paper: